Journal: Cell Death & Disease

Article Title: Alox8 knockout exacerbates imiquimod-induced psoriasis-like inflammation

doi: 10.1038/s41419-026-08727-9

Figure Lengend Snippet: A Representative images of Alox8 RNA detected by BaseScope™ in situ hybridisation (fast red) in murine tissue array, nuclei counterstained with haematoxylin (blue). Scale bars 100 μm or zoomed regions 20 μm. B Quantification of subcellular spot detection per cm 2 of tissue detected per tissue microarray core. C Gene expression analysis of Alox8 in RNA extracted from bulk tissue of WT and Alox8 KO mice, relative to 18S . Data are mean ± SEM, N = 2-3 for B and N = 3 for C .

Article Snippet: In situ hybridisation was performed with a mouse multiple normal organ tissue array (MOJ6221a-BX, Bio-Cat) with probe mm-Alox8 (1089301-C1, ACD-Bio) and BaseScope Assay Red kit (ACD-Bio) as per manufacturer’s recommendations.

Techniques: In Situ, Hybridization, Microarray, Gene Expression

Journal: Cancer research

Article Title: IL-26, a non-canonical mediator of DNA inflammatory stimulation, promotes TNBC engraftment and progression in association with neutrophils.

doi: 10.1158/0008-5472.CAN-18-3825

Figure Lengend Snippet: A) Stratifying TCGA BC dataset by molecular subtype reveals that Basal-like tumors are significantly enriched for IL-26 compared to other molecular subtypes (Total= 981 BCs; Luminal A n=499; Luminal B n=197; Her2-enriched n=78; Normal-like n=36; Basal-like n=171). B) RNAscope analysis of a BC Progression Tissue Microarray for IL-26 mRNA detected significantly more IL-26 transcripts compared to all other BCs (Non-TNBC n=107; TNBC n=23). C) Representative images of RNAscope results on tissue microarrays for both TNBC and Non-TNBC tumors. Tumor cell clusters are indicated with dashed lines. Nuclei (Dapi) are indicated in grey and IL-26 is indicated by yellow. D) Single-cell RNA profiling of TNBC patients (GSE110686), Group 6 indicated. E) Expression profile of IL-26 among different CD45+ groups identified in patient TNBC samples. Bars indicate SEM and ** p < 0.01, **** p < 0.0001.

Article Snippet: RNA in situ hybridization (RNAscope): In situ detection of IL26 mRNA transcripts in was performed on formalin-fixed, paraffin-embedded Cancer Diagnosis Program (CDP) Breast Cancer Progression Tissue Microarray sets using the RNAscope Multiplex Fluorescent kit (323100; Advanced Cell Diagnostics, Newark, CA)( 28 ) following the manufacturer’s protocol.

Techniques: RNAscope, Microarray, Single Cell, Expressing